CA19-9 Antibody & ELISA
Explore how ELISA works and how Anogen’s CA19-9 antibodies and ELISA kit provide high specificity, reproducibility, and reliable biomarker quantification for research.
ELISA-Based Quantification of CA19-9 Using Matched Monoclonal Antibodies and High-Performance Immunoassay Systems
Carbohydrate Antigen 19-9 (CA19-9) (Wikipedia), also known as sialyl-Lewisᵃ, is widely studied as a serum-associated biomarker in pancreatic and gastrointestinal research. Accurate assessment of CA19-9 levels requires immunoassay systems with high analytical specificity, reproducibility, and stability.
Enzyme-Linked Immunosorbent Assay (ELISA) remains one of the most versatile analytical platforms for detecting and quantifying proteins, glycoproteins, peptides, and carbohydrate antigens in biological research. Its modular design, high sensitivity, and compatibility with a broad range of samples make ELISA an indispensable technique in immunochemistry and protein analytics.
Among the various immunoassay formats, the sandwich ELISA is particularly valued for its selectivity. By employing two independent monoclonal antibodies targeting non-overlapping epitopes, the assay achieves enhanced specificity, reduced background signal, and reproducible readouts. This makes it especially suitable for studies of biomolecules that exist at low concentrations or within complex biological matrices.
Scientific Background of CA19-9
CA19-9 (sialyl-Lewisᵃ) is a tetrasaccharide-terminated glycan expressed on mucin-type glycoproteins and cell-surface glycolipids. It is frequently investigated in the context of gastrointestinal biology, carbohydrate antigen expression, and mucin-associated molecular pathways. Because CA19-9 is linked to glycosylation patterns and secretory processes, its quantification plays a key role in glycobiology studies, biomarker development, and research involving epithelial cell behavior. ScienceDirecte
Given its biochemical nature and distribution, CA19-9 requires immunological detection systems that can reliably recognize the epitope without cross-reactivity to structurally similar glycans. This places substantial emphasis on the specificity and affinity of the monoclonal antibodies used in the assay.
Matched Monoclonal Antibodies for Sandwich ELISA
Anogen’s CA19-9 research tools include a validated monoclonal antibody pair optimized for sandwich ELISA applications :
Capture antibody
Detection antibody
These antibodies are engineered to bind distinct regions of the CA19-9 epitope, enabling the formation of a highly selective antibody–antigen–antibody complex. The pair exhibits :
- High lot-to-lot consistency.
- Strong analytical specificity.
- Minimal background signal.
- Reliable performance across ELISA and IHC applications.
This matched system forms the basis of a robust analytical platform for CA19-9 quantification.
Human CA19-9 ELISA Kit
Building on the performance of Clones 4E11 and 9B2, the Human CA19-9 ELISA Kit provides a ready-to-use system for precise CA19-9 measurement in research settings. Technical highlights include:
- Detection limit : 1.7 U/mL
- Coefficient of variation (CV) : < 6%
- Assay time : ~2.5 hours
- Dynamic range optimized for quantitative applications
- Reproducible calibration curve with reliable inter-assay consistency
The kit includes pre-coated plates, standards, detection reagents, substrate, stop solution, and a validated protocol, supporting fast and reproducible experimental workflows.
Applications in Research
Glycobiology and Carbohydrate Antigen Research
CA19-9 serves as a model glycan-associated antigen for studying carbohydrate-protein interactions.
Immunochemistry and ELISA Development
The stable performance of the 4E11/9B2 system makes it a valuable reference for developing and optimizing sandwich ELISA methodologies.
Cell-Surface Glycosylation Studies
CA19-9 is frequently studied in relation to mucin biochemistry, membrane trafficking, and secretory pathways.
Assay Validation and Analytical Methodology
Because its structure and behavior are well characterized, CA19-9 is useful for validating detection platforms and establishing internal quality controls.
The integration of high-quality monoclonal antibodies and well-characterized assay components is essential for generating reproducible and meaningful data in biomarker and glycobiology research. Anogen’s CA19-9 antibodies (Clones 4E11 and 9B2) and Human CA19-9 ELISA Kit provide a validated, precise, and efficient platform for the quantitative study of CA19-9.
By combining strong specificity, reproducible performance, and optimized assay design, these tools support ongoing investigations into carbohydrate antigens, glycoprotein expression, and immunochemical analytics across many scientific disciplines.
Our CA19-9 Antibody & ELISA
Perfect for laboratories needing accurate, simple, and dependable CA19-9 measurement tools.