D-22 Insect Medium without Sodium bicarbonate

Without: Sodium bicarbonate D-22 medium (1976) is the final modification of the original D20 medium developed by Echalier and Ohanessian (1970). The medium composition closely initiates the principal characteristics of the body fluid of Drosophila 3rd instar larvae. This medium has been extensively used to grow many established Drosophila cell lines, including the famous Kc cell line. IM005 is D-22 medium . It is a mixture of inorganic anions like sodium and potassium glutamate, glycine and Kreb's cycle intermediates. The medium also contains lactalbumin hydrolysate which provides large number of free amino acids. The medium is further made nutritionally rich by extract and vitamins. Supplemented with 5 -20% fetal bovine serum, this medium can be used for the maintenance of primary and established cell lines desired from Drosophila melanogaster. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.