Ruminant Pregnancy Rapid Test Kit-by whole blood- 10 tests

Product Name: Ruminant Early Pregnancy Rapid Test Kit

Purpose: Identifying non-pregnant ruminant animals 28 days after fertilization

Test Type: Lateral Flow Assay

Sample Types: Whole Blood (EDTA), Serum, or Plasma

Packaging: 10 tests per box

Kit Components:

• 10 individually packaged test cards with sample pipettes
• 1 bottle of sample diluent (3 ml)
• 1 instruction manual
  Key Features:
• Early Detection: Detects pregnancy as early as 28 days post-breeding
• User-Friendly: No additional equipment required
• High Accuracy: 99.5%
• Rapid Results: Results available in 20 minutes
  Storage Conditions: Store at 2–30°C; protect from direct sunlight, moisture, and heat
  Shelf Life: 24 months​

Overview:

Timely and accurate pregnancy detection in ruminants—such as cattle, goats, and sheep—is vital for effective herd reproductive management. Early identification of non-pregnant animals allows for prompt rebreeding, reducing calving intervals and enhancing overall farm productivity.​

This rapid test employs a double-antibody sandwich method combined with colloidal gold chromatography to detect pregnancy-associated glycoproteins (PAGs), which are specific markers produced by the placenta during pregnancy.

• In dairy cows as early as 28 days post-breeding and for cows over 60 days postpartum
• In goats starting from 28 days post-breeding
• In sheep from 35 days post-breeding​

Detection Principle:

Upon adding the sample to the test card, any PAGs present bind to gold-labeled antibodies, forming a complex. This complex migrates along the test strip and is captured by antibodies immobilized on the test line (T line), resulting in a visible red band. The intensity of the band correlates with the concentration of PAGs. A control line (C line) should always appear to confirm the test's validity.​

Test Procedure:

1. Preparation:
   • Ensure all test components and samples are at room temperature (15–35°C) before starting.​
2. Sample Collection:
   • Whole Blood: Collect blood in EDTA tubes and mix gently by inverting the tube 10 times. Store at 2–8°C for up to 48 hours. Do not freeze.
   • Serum/Plasma: Centrifuge collected blood at 2000–3000 rpm for 5–15 minutes or allow it to stand until separation occurs. Store at 2–8°C for up to 7 days.​
3. Testing:
   • Open the foil pouch and place the test card on a flat surface.
   • Apply 2 drops (~50 µL) of the sample to the sample well.
   • Add 2 drops (~50 µL) of sample diluent to the same well.
   • Start a timer for 20 minutes.
   • Interpret the results:
     • Positive: Both T and C lines appear.
     • Negative: Only the C line appears.
     • Invalid: No lines appear or only the T line appears.

Note: Even a faint T line indicates a positive result.