Schneider’s Insect Medium with L-Glutamine, w/o Calcium chloride and Sodium bicarbonate

With: L-Glutamine Without: Calcium chloride and Sodium bicarbonate Schneider's insect medium has been specially formulated for the in vitro culture of Drosophila melanogaster cells and tissues. A number of cell lines derived from Drosophila melanogaster are now available and are extensively used in genetic and molecular biology research. Drosophila cells are also used to study recombinant protein expression. IM003 is Schneider's insect medium with L-glutamine. It does not contain calcium chloride and sodium bicarbonate, hence needs to be supplemented with calcium chloride and sodium bicarbonate while preparing the complete medium. The basal medium also needs to be supplemented with 5 - 20% of heat inactivated fetal bovine serum to supply the necessary growth factors to the insect cells. Schneider's insect medium is an extremely nutritious medium and supports rapid growth of cells derived from Drosophila. It can also be used to culture cell lines derived from other dipteran species. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.