TC-100 Insect Medium with L-Glutamine, w/o Sodium bicarbonate

With: L-Glutamine Without: Sodium bicarbonate TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done tooptimise the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. IM007 is TC-100 Insect Medium with L-glutamine. TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.