TNM-FH Insect Medium with L-Glutamine, Lactalbumin hydrolysate and Extract w/o Sodium bicarbonate

With: L-Glutamine Without: Sodium bicarbonate TNM-FH medium is a modification of Grace's insect medium. The modification was developed by Dr. W. F. Hink to grow Cabbage looper cell line TN-368. Presently, this medium is used to grow cells derived from a variety of lepidopterans species. IM008 , TNM-FH insect medium, is Grace's medium supplemented with lactalbumin hydrolysate and yeast extract. Lactalbumin hydrolysate provides a large number of free amino acids. extract serves as a source of vitamins. Supplemented with 5- 20% fetal bovine serum this medium supports growth of number of insect cell lines derived from Lepidopteran species. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.